Analytical techniques for cell fractions. I. Simplified gradient elution programming.

Many methods have been devised for producing gradients in elution fluids for ion-exchange chromatography (l-9). While many of these are invaluable for special purposes, none appears to allow: (a) the production of a complex multicomponent gradient directly from a graphic presentation, and (b) the possibility of changing from gradient to stepwise elution with the same system. A simple method for producing complex gradients or stepwise elutions is described.